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cdc25c  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cdc25c
    Cdc25c, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdc25c/Phospho-cdc25C+(Ser216)+Rabbit+mAb/pmc13003987-89-45-46
    Average 94 stars, based on 128 article reviews
    cdc25c - by Bioz Stars, 2026-11
    94/100 stars

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    Cell-Signaling:

    Article Title: The impact of evodiamine on human anaplastic thyroid cancer therapy—an in vitro and in vivo study
    Article Snippet: .. The primary antibodies used in this study included: cdc2 (#9116), cdc25C (#4688), p21 (#2947), caspase-3 (#9662), caspase-9 (#9502), and PARP (#9542) (from Cell Signaling Technology, Danvers, MA, USA), and cyclin A (GTX27956), cyclin B1 (GTX100911), and GAPDH (GTX100118) (from GeneTex, Hsinchu City, Taiwan). .. To confirm the involvement of caspase activation in EVO-induced apoptosis, cells were pre-treated with the pan-caspase inhibitor Z-VAD-FMK (BioVision, Mountain View, CA, USA).

    Article Title: Scutebarbatine B Exerts Anti-Breast Cancer Activity by Inducing Cell Cycle Arrest and Apoptosis Through Multiple Pathways.
    Article Snippet: Breast cancer is the most commonly occurring cancer among women with high mortality.. Identifying effective anticancer compounds to improve the overall survival is imperative.. The present study was designed to evaluate the effects and underlying mechanisms of Scutebarbatine B (SBTB), a diterpenoid alkaloid extracted from Scutellaria barbata D. Don (S. barbata), on breast cancer.

    Article Title: The Effect of AZD5153 on Radiosensitivity in Pancreatic Cancer Cells Through ATM-chk1 Pathway
    Article Snippet: .. The membranes were blocked with 5% skim milk for 2 h, and incubated with primary antibodies against BRD4 (Cell Signaling Technology, Cat# 13440s), c-Myc (Cell Signaling Technology, Cat# 18583s), p-ATM (Abcam, Cat# ab81292), ATM (Cell Signaling Technology, Cat# 2873s), p-cdc25C (Cell Signaling Technology, Cat# 4901s), cdc25C (Cell Signaling Technology, Cat# 4688s), p-chk1 (Cell Signaling Technology, Cat# 2348s), chk1 (Abcam, Cat# ab40866), p-cdc2 (Cell Signaling Technology, Cat# 4539s), cdc2 (Cell Signaling Technology, Cat# 9116s), γ-H2AX (Cell Signaling Technology, Cat# 9718s), cleaved PARP (Cell Signaling Technology, Cat# 5625s), Bax (Cell Signaling Technology, Cat# 14796s), β-actin (Abcam, Cat# ab6276),Vinculin (Cell Signaling Technology, Cat# 13901s) overnight at 4 °C. .. After washing with Tris buffered saline with Tween 20 (TBST) for 3 times, the membrane followed by incubation with the appropriate HRP-conjugated secondary antibody (Beyotime, Cat# A0208/Cat# A0216) for 1 h at room temperature.

    other:

    Article Title: Deoxypodophyllotoxin inhibited the growth of malignant pleural mesothelioma by inducing necroptosis and mitotic catastrophe.
    Article Snippet: Background: Malignant pleural mesothelioma (MPM) is an extremely aggressive cancer with a poor prognosis and limited effective treatment options.. However, recent studies have shown that targeting microtubule regulation is a viable approach for treating MPM.. Purpose: This study aimed to assess the antitumor behavior of deoxypodophyllotoxin (DPT) on MPM in vitro and in vivo and to identify its underlying mechanisms.


    Incubation:

    Article Title: The Effect of AZD5153 on Radiosensitivity in Pancreatic Cancer Cells Through ATM-chk1 Pathway
    Article Snippet: .. The membranes were blocked with 5% skim milk for 2 h, and incubated with primary antibodies against BRD4 (Cell Signaling Technology, Cat# 13440s), c-Myc (Cell Signaling Technology, Cat# 18583s), p-ATM (Abcam, Cat# ab81292), ATM (Cell Signaling Technology, Cat# 2873s), p-cdc25C (Cell Signaling Technology, Cat# 4901s), cdc25C (Cell Signaling Technology, Cat# 4688s), p-chk1 (Cell Signaling Technology, Cat# 2348s), chk1 (Abcam, Cat# ab40866), p-cdc2 (Cell Signaling Technology, Cat# 4539s), cdc2 (Cell Signaling Technology, Cat# 9116s), γ-H2AX (Cell Signaling Technology, Cat# 9718s), cleaved PARP (Cell Signaling Technology, Cat# 5625s), Bax (Cell Signaling Technology, Cat# 14796s), β-actin (Abcam, Cat# ab6276),Vinculin (Cell Signaling Technology, Cat# 13901s) overnight at 4 °C. .. After washing with Tris buffered saline with Tween 20 (TBST) for 3 times, the membrane followed by incubation with the appropriate HRP-conjugated secondary antibody (Beyotime, Cat# A0208/Cat# A0216) for 1 h at room temperature.

    Article Title: The Effect of AZD5153 on Radiosensitivity in Pancreatic Cancer Cells Through ATM-chk1 Pathway
    Article Snippet: .. The membranes were blocked with 5% skim milk for 2 h, and incubated with primary antibodies against BRD4 (Cell Signaling Technology, Cat# 13440s), c-Myc (Cell Signaling Technology, Cat# 18583s), p-ATM (Abcam, Cat# ab81292), ATM (Cell Signaling Technology, Cat# 2873s), p-cdc25C (Cell Signaling Technology, Cat# 4901s), cdc25C (Cell Signaling Technology, Cat# 4688s), p-chk1 (Cell Signaling Technology, Cat# 2348s), chk1 (Abcam, Cat# ab40866), p-cdc2 (Cell Signaling Technology, Cat# 4539s), cdc2 (Cell Signaling Technology, Cat# 9116s), γ-H2AX (Cell Signaling Technology, Cat# 9718s), cleaved PARP (Cell Signaling Technology, Cat# 5625s), Bax (Cell Signaling Technology, Cat# 14796s), β-actin (Abcam, Cat# ab6276),Vinculin (Cell Signaling Technology, Cat# 13901s) overnight at 4 °C. .. After washing with Tris buffered saline with Tween 20 (TBST) for 3 times, the membrane followed by incubation with the appropriate HRP-conjugated secondary antibody (Beyotime, Cat# A0208/Cat# A0216) for 1 h at room temperature.



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    TNKS regulates BubR1/MPF activity, and Securin accumulation in mouse oocyte. (A) Representative images of BubR1 signals at the kinetochore in oocytes from the control and JW55 treatment groups. Green, BubR1. Red, ACA. Blue, DNA. Bar = 5 μm. (B) The percentage of positive BubR1 signals at the kinetochore in oocytes from the control (n = 53) and JW55 (n = 50) treatment groups. ***, P < 0.001. (C) Representative images of oocytes PB1 extrusion after 12 h culture in the Control, control + Milri, control + 10 μM ETOP, JW55 + 10 μM ETOP and JW55 groups. GV oocytes were exposed to etoposide (ETOP) at concentrations of 1 μM and 10 μM for 30 min in the presence 4 μM milrinone (Milri) to prevent GVBD during this treatment. Bar = 80 μm. (D) The percentage of PB1 or large PB1 extrusion in the control (n = 94), control + Milri (n = 89), control + 1 μM ETOP (n = 98), control + 10 μM ETOP (n = 97), JW55 + 1 μ M ETOP (n = 128), JW55 + 10 μM ETOP (n = 120), and JW55 treatment (n = 114) oocytes after ETOP pretreatment. ns, P > 0.05. **, P < 0.01. ***, P < 0.001. (E) Representative images of γ-H2AX in MI stage oocyte from the control + 10 μM ETOP and JW55 + 10 μM ETOP oocytes. Red, γ-H2AX. Blue, DNA. Bar = 5 μm. (F) Relative intensity of γ-H2AX in MI stage oocyte from the control + 10 μM ETOP (n = 47) and JW55 + 10 μM ETOP (n = 39) oocytes. ***, P < 0.001. (G) Western blot of Cyclin B1 and Securin in oocytes from the control and JW55 treatment groups after 6 h of culture. (H) Western blot of pT161-CDK1 and CDC20 in oocytes from the control and JW55 treatment groups after 6 h of culture, and band intensity analysis of their protein levels in oocytes from the control and JW55 treatment groups. *, P < 0.05. **, P < 0.01. (I) Band intensity analysis of pT161-CDK1, pY15-CDK1, and Securin in oocytes from the control and JW55 treatment groups after 3 h of culture. ns, P > 0.05. *, P < 0.05. (J) Band intensity analysis of <t>CDC25C,</t> Cyclin B1, and pT14-CDK1 in oocytes from the control and JW55 treatment groups after 3 h of culture. *, P < 0.05. **, P < 0.01. (K) Protein-protein interaction network analysis of cell cycle-related proteins identified by mass spectrometry and MPF/Securin/CDC20/CDC25C upon STRING database. Different node color indicated core and non-core nodes, while edge thickness edge represented interaction strength. (L) Co-IP analysis with an anti-TNKS antibody. The immunoblot was probed with anti-CDC20 and anti-CDC25C antibodies. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
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    TNKS regulates BubR1/MPF activity, and Securin accumulation in mouse oocyte. (A) Representative images of BubR1 signals at the kinetochore in oocytes from the control and JW55 treatment groups. Green, BubR1. Red, ACA. Blue, DNA. Bar = 5 μm. (B) The percentage of positive BubR1 signals at the kinetochore in oocytes from the control (n = 53) and JW55 (n = 50) treatment groups. ***, P < 0.001. (C) Representative images of oocytes PB1 extrusion after 12 h culture in the Control, control + Milri, control + 10 μM ETOP, JW55 + 10 μM ETOP and JW55 groups. GV oocytes were exposed to etoposide (ETOP) at concentrations of 1 μM and 10 μM for 30 min in the presence 4 μM milrinone (Milri) to prevent GVBD during this treatment. Bar = 80 μm. (D) The percentage of PB1 or large PB1 extrusion in the control (n = 94), control + Milri (n = 89), control + 1 μM ETOP (n = 98), control + 10 μM ETOP (n = 97), JW55 + 1 μ M ETOP (n = 128), JW55 + 10 μM ETOP (n = 120), and JW55 treatment (n = 114) oocytes after ETOP pretreatment. ns, P > 0.05. **, P < 0.01. ***, P < 0.001. (E) Representative images of γ-H2AX in MI stage oocyte from the control + 10 μM ETOP and JW55 + 10 μM ETOP oocytes. Red, γ-H2AX. Blue, DNA. Bar = 5 μm. (F) Relative intensity of γ-H2AX in MI stage oocyte from the control + 10 μM ETOP (n = 47) and JW55 + 10 μM ETOP (n = 39) oocytes. ***, P < 0.001. (G) Western blot of Cyclin B1 and Securin in oocytes from the control and JW55 treatment groups after 6 h of culture. (H) Western blot of pT161-CDK1 and CDC20 in oocytes from the control and JW55 treatment groups after 6 h of culture, and band intensity analysis of their protein levels in oocytes from the control and JW55 treatment groups. *, P < 0.05. **, P < 0.01. (I) Band intensity analysis of pT161-CDK1, pY15-CDK1, and Securin in oocytes from the control and JW55 treatment groups after 3 h of culture. ns, P > 0.05. *, P < 0.05. (J) Band intensity analysis of <t>CDC25C,</t> Cyclin B1, and pT14-CDK1 in oocytes from the control and JW55 treatment groups after 3 h of culture. *, P < 0.05. **, P < 0.01. (K) Protein-protein interaction network analysis of cell cycle-related proteins identified by mass spectrometry and MPF/Securin/CDC20/CDC25C upon STRING database. Different node color indicated core and non-core nodes, while edge thickness edge represented interaction strength. (L) Co-IP analysis with an anti-TNKS antibody. The immunoblot was probed with anti-CDC20 and anti-CDC25C antibodies. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
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    TNKS regulates BubR1/MPF activity, and Securin accumulation in mouse oocyte. (A) Representative images of BubR1 signals at the kinetochore in oocytes from the control and JW55 treatment groups. Green, BubR1. Red, ACA. Blue, DNA. Bar = 5 μm. (B) The percentage of positive BubR1 signals at the kinetochore in oocytes from the control (n = 53) and JW55 (n = 50) treatment groups. ***, P < 0.001. (C) Representative images of oocytes PB1 extrusion after 12 h culture in the Control, control + Milri, control + 10 μM ETOP, JW55 + 10 μM ETOP and JW55 groups. GV oocytes were exposed to etoposide (ETOP) at concentrations of 1 μM and 10 μM for 30 min in the presence 4 μM milrinone (Milri) to prevent GVBD during this treatment. Bar = 80 μm. (D) The percentage of PB1 or large PB1 extrusion in the control (n = 94), control + Milri (n = 89), control + 1 μM ETOP (n = 98), control + 10 μM ETOP (n = 97), JW55 + 1 μ M ETOP (n = 128), JW55 + 10 μM ETOP (n = 120), and JW55 treatment (n = 114) oocytes after ETOP pretreatment. ns, P > 0.05. **, P < 0.01. ***, P < 0.001. (E) Representative images of γ-H2AX in MI stage oocyte from the control + 10 μM ETOP and JW55 + 10 μM ETOP oocytes. Red, γ-H2AX. Blue, DNA. Bar = 5 μm. (F) Relative intensity of γ-H2AX in MI stage oocyte from the control + 10 μM ETOP (n = 47) and JW55 + 10 μM ETOP (n = 39) oocytes. ***, P < 0.001. (G) Western blot of Cyclin B1 and Securin in oocytes from the control and JW55 treatment groups after 6 h of culture. (H) Western blot of pT161-CDK1 and CDC20 in oocytes from the control and JW55 treatment groups after 6 h of culture, and band intensity analysis of their protein levels in oocytes from the control and JW55 treatment groups. *, P < 0.05. **, P < 0.01. (I) Band intensity analysis of pT161-CDK1, pY15-CDK1, and Securin in oocytes from the control and JW55 treatment groups after 3 h of culture. ns, P > 0.05. *, P < 0.05. (J) Band intensity analysis of <t>CDC25C,</t> Cyclin B1, and pT14-CDK1 in oocytes from the control and JW55 treatment groups after 3 h of culture. *, P < 0.05. **, P < 0.01. (K) Protein-protein interaction network analysis of cell cycle-related proteins identified by mass spectrometry and MPF/Securin/CDC20/CDC25C upon STRING database. Different node color indicated core and non-core nodes, while edge thickness edge represented interaction strength. (L) Co-IP analysis with an anti-TNKS antibody. The immunoblot was probed with anti-CDC20 and anti-CDC25C antibodies. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
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    Effect of AZD5153 on protein expression in irradiated pancreatic cancer cells, n=6. ( a and b ) Western blot analysis was performed to detect the expression of BRD4, c-Myc in different groups, β-actin was used as a loading control. ( c and d ) The protein expression of p-ATM, ATM, <t>p-chk1,</t> chk1, p-cdc25, cdc25, p-cdc2, cdc2 in different groups was detected by western blot, β-actin was used as a loading control. Results shown are the means ± SD of 3 independent experiments with similar results for all assays. Significance was determined by Student’s t -test (* p < 0.05, ** p < 0.01, *** p < 0.001).
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    Image Search Results


    TNKS regulates BubR1/MPF activity, and Securin accumulation in mouse oocyte. (A) Representative images of BubR1 signals at the kinetochore in oocytes from the control and JW55 treatment groups. Green, BubR1. Red, ACA. Blue, DNA. Bar = 5 μm. (B) The percentage of positive BubR1 signals at the kinetochore in oocytes from the control (n = 53) and JW55 (n = 50) treatment groups. ***, P < 0.001. (C) Representative images of oocytes PB1 extrusion after 12 h culture in the Control, control + Milri, control + 10 μM ETOP, JW55 + 10 μM ETOP and JW55 groups. GV oocytes were exposed to etoposide (ETOP) at concentrations of 1 μM and 10 μM for 30 min in the presence 4 μM milrinone (Milri) to prevent GVBD during this treatment. Bar = 80 μm. (D) The percentage of PB1 or large PB1 extrusion in the control (n = 94), control + Milri (n = 89), control + 1 μM ETOP (n = 98), control + 10 μM ETOP (n = 97), JW55 + 1 μ M ETOP (n = 128), JW55 + 10 μM ETOP (n = 120), and JW55 treatment (n = 114) oocytes after ETOP pretreatment. ns, P > 0.05. **, P < 0.01. ***, P < 0.001. (E) Representative images of γ-H2AX in MI stage oocyte from the control + 10 μM ETOP and JW55 + 10 μM ETOP oocytes. Red, γ-H2AX. Blue, DNA. Bar = 5 μm. (F) Relative intensity of γ-H2AX in MI stage oocyte from the control + 10 μM ETOP (n = 47) and JW55 + 10 μM ETOP (n = 39) oocytes. ***, P < 0.001. (G) Western blot of Cyclin B1 and Securin in oocytes from the control and JW55 treatment groups after 6 h of culture. (H) Western blot of pT161-CDK1 and CDC20 in oocytes from the control and JW55 treatment groups after 6 h of culture, and band intensity analysis of their protein levels in oocytes from the control and JW55 treatment groups. *, P < 0.05. **, P < 0.01. (I) Band intensity analysis of pT161-CDK1, pY15-CDK1, and Securin in oocytes from the control and JW55 treatment groups after 3 h of culture. ns, P > 0.05. *, P < 0.05. (J) Band intensity analysis of CDC25C, Cyclin B1, and pT14-CDK1 in oocytes from the control and JW55 treatment groups after 3 h of culture. *, P < 0.05. **, P < 0.01. (K) Protein-protein interaction network analysis of cell cycle-related proteins identified by mass spectrometry and MPF/Securin/CDC20/CDC25C upon STRING database. Different node color indicated core and non-core nodes, while edge thickness edge represented interaction strength. (L) Co-IP analysis with an anti-TNKS antibody. The immunoblot was probed with anti-CDC20 and anti-CDC25C antibodies. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Journal: Journal of Advanced Research

    Article Title: Tankyrase activity is essential for asymmetric division and chromosome segregation in oocyte meiosis

    doi: 10.1016/j.jare.2025.07.008

    Figure Lengend Snippet: TNKS regulates BubR1/MPF activity, and Securin accumulation in mouse oocyte. (A) Representative images of BubR1 signals at the kinetochore in oocytes from the control and JW55 treatment groups. Green, BubR1. Red, ACA. Blue, DNA. Bar = 5 μm. (B) The percentage of positive BubR1 signals at the kinetochore in oocytes from the control (n = 53) and JW55 (n = 50) treatment groups. ***, P < 0.001. (C) Representative images of oocytes PB1 extrusion after 12 h culture in the Control, control + Milri, control + 10 μM ETOP, JW55 + 10 μM ETOP and JW55 groups. GV oocytes were exposed to etoposide (ETOP) at concentrations of 1 μM and 10 μM for 30 min in the presence 4 μM milrinone (Milri) to prevent GVBD during this treatment. Bar = 80 μm. (D) The percentage of PB1 or large PB1 extrusion in the control (n = 94), control + Milri (n = 89), control + 1 μM ETOP (n = 98), control + 10 μM ETOP (n = 97), JW55 + 1 μ M ETOP (n = 128), JW55 + 10 μM ETOP (n = 120), and JW55 treatment (n = 114) oocytes after ETOP pretreatment. ns, P > 0.05. **, P < 0.01. ***, P < 0.001. (E) Representative images of γ-H2AX in MI stage oocyte from the control + 10 μM ETOP and JW55 + 10 μM ETOP oocytes. Red, γ-H2AX. Blue, DNA. Bar = 5 μm. (F) Relative intensity of γ-H2AX in MI stage oocyte from the control + 10 μM ETOP (n = 47) and JW55 + 10 μM ETOP (n = 39) oocytes. ***, P < 0.001. (G) Western blot of Cyclin B1 and Securin in oocytes from the control and JW55 treatment groups after 6 h of culture. (H) Western blot of pT161-CDK1 and CDC20 in oocytes from the control and JW55 treatment groups after 6 h of culture, and band intensity analysis of their protein levels in oocytes from the control and JW55 treatment groups. *, P < 0.05. **, P < 0.01. (I) Band intensity analysis of pT161-CDK1, pY15-CDK1, and Securin in oocytes from the control and JW55 treatment groups after 3 h of culture. ns, P > 0.05. *, P < 0.05. (J) Band intensity analysis of CDC25C, Cyclin B1, and pT14-CDK1 in oocytes from the control and JW55 treatment groups after 3 h of culture. *, P < 0.05. **, P < 0.01. (K) Protein-protein interaction network analysis of cell cycle-related proteins identified by mass spectrometry and MPF/Securin/CDC20/CDC25C upon STRING database. Different node color indicated core and non-core nodes, while edge thickness edge represented interaction strength. (L) Co-IP analysis with an anti-TNKS antibody. The immunoblot was probed with anti-CDC20 and anti-CDC25C antibodies. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Rabbit anti-α-Tubulin antibody (11224-1-AP), rabbit polyclonal anti-GAPDH antibody (10494-1-AP), rabbit anti-GRP78 antibody (11587-1-AP), rabbit anti-TNKS1 antibody (18030-1-AP), rabbit anti-Ran antibody (10469-1-AP), and rabbit anti-Rab11a antibody (67902-1-Ig), rabbit anti-Securin antibody (18040-1-AP), rabbit anti-CDC25C antibody (66912-1-Ig), rabbit anti-Formin2 antibody (11259-1-AP), rabbit anti-BubR1 (11504-2-AP) were from Proteintech.

    Techniques: Activity Assay, Control, Western Blot, Mass Spectrometry, Co-Immunoprecipitation Assay

    Effect of AZD5153 on protein expression in irradiated pancreatic cancer cells, n=6. ( a and b ) Western blot analysis was performed to detect the expression of BRD4, c-Myc in different groups, β-actin was used as a loading control. ( c and d ) The protein expression of p-ATM, ATM, p-chk1, chk1, p-cdc25, cdc25, p-cdc2, cdc2 in different groups was detected by western blot, β-actin was used as a loading control. Results shown are the means ± SD of 3 independent experiments with similar results for all assays. Significance was determined by Student’s t -test (* p < 0.05, ** p < 0.01, *** p < 0.001).

    Journal: Drug Design, Development and Therapy

    Article Title: The Effect of AZD5153 on Radiosensitivity in Pancreatic Cancer Cells Through ATM-chk1 Pathway

    doi: 10.2147/DDDT.S568551

    Figure Lengend Snippet: Effect of AZD5153 on protein expression in irradiated pancreatic cancer cells, n=6. ( a and b ) Western blot analysis was performed to detect the expression of BRD4, c-Myc in different groups, β-actin was used as a loading control. ( c and d ) The protein expression of p-ATM, ATM, p-chk1, chk1, p-cdc25, cdc25, p-cdc2, cdc2 in different groups was detected by western blot, β-actin was used as a loading control. Results shown are the means ± SD of 3 independent experiments with similar results for all assays. Significance was determined by Student’s t -test (* p < 0.05, ** p < 0.01, *** p < 0.001).

    Article Snippet: The membranes were blocked with 5% skim milk for 2 h, and incubated with primary antibodies against BRD4 (Cell Signaling Technology, Cat# 13440s), c-Myc (Cell Signaling Technology, Cat# 18583s), p-ATM (Abcam, Cat# ab81292), ATM (Cell Signaling Technology, Cat# 2873s), p-cdc25C (Cell Signaling Technology, Cat# 4901s), cdc25C (Cell Signaling Technology, Cat# 4688s), p-chk1 (Cell Signaling Technology, Cat# 2348s), chk1 (Abcam, Cat# ab40866), p-cdc2 (Cell Signaling Technology, Cat# 4539s), cdc2 (Cell Signaling Technology, Cat# 9116s), γ-H2AX (Cell Signaling Technology, Cat# 9718s), cleaved PARP (Cell Signaling Technology, Cat# 5625s), Bax (Cell Signaling Technology, Cat# 14796s), β-actin (Abcam, Cat# ab6276),Vinculin (Cell Signaling Technology, Cat# 13901s) overnight at 4 °C.

    Techniques: Expressing, Irradiation, Western Blot, Control

    The effect of overexpression of chk1 on enhancing the radiosensitivity of AZD5153, n=6. ( a ) Overexpression of chk1 protein in Capan2 cells by chk1 plasmid; ( b ) Quantitative statistics of apoptosis in Capan2 cells caused by chk1 overexpression; ( c ) Representative results of the effect of chk1 overexpression on apoptosis of Capan2 cells. Results shown are the means ± SD of 3 independent experiments with similar results for all assays. Significance was determined by Student’s t -test (* p < 0.05, *** p < 0.001).

    Journal: Drug Design, Development and Therapy

    Article Title: The Effect of AZD5153 on Radiosensitivity in Pancreatic Cancer Cells Through ATM-chk1 Pathway

    doi: 10.2147/DDDT.S568551

    Figure Lengend Snippet: The effect of overexpression of chk1 on enhancing the radiosensitivity of AZD5153, n=6. ( a ) Overexpression of chk1 protein in Capan2 cells by chk1 plasmid; ( b ) Quantitative statistics of apoptosis in Capan2 cells caused by chk1 overexpression; ( c ) Representative results of the effect of chk1 overexpression on apoptosis of Capan2 cells. Results shown are the means ± SD of 3 independent experiments with similar results for all assays. Significance was determined by Student’s t -test (* p < 0.05, *** p < 0.001).

    Article Snippet: The membranes were blocked with 5% skim milk for 2 h, and incubated with primary antibodies against BRD4 (Cell Signaling Technology, Cat# 13440s), c-Myc (Cell Signaling Technology, Cat# 18583s), p-ATM (Abcam, Cat# ab81292), ATM (Cell Signaling Technology, Cat# 2873s), p-cdc25C (Cell Signaling Technology, Cat# 4901s), cdc25C (Cell Signaling Technology, Cat# 4688s), p-chk1 (Cell Signaling Technology, Cat# 2348s), chk1 (Abcam, Cat# ab40866), p-cdc2 (Cell Signaling Technology, Cat# 4539s), cdc2 (Cell Signaling Technology, Cat# 9116s), γ-H2AX (Cell Signaling Technology, Cat# 9718s), cleaved PARP (Cell Signaling Technology, Cat# 5625s), Bax (Cell Signaling Technology, Cat# 14796s), β-actin (Abcam, Cat# ab6276),Vinculin (Cell Signaling Technology, Cat# 13901s) overnight at 4 °C.

    Techniques: Over Expression, Plasmid Preparation